Western Blot:Article Title: KDM3B inhibitors disrupt the oncogenic activity of PAX3-FOXO1 in fusion-positive rhabdomyosarcoma
Article Snippet: H3K9me2, Abcam, ab1220, Western, ChIP, 1:5000, 0.03 μg/μL H3K4me3, Active motif, 39159, Western, ChIP, 1:4000, 0.03 μg/μL H3K27me3, Active motif, 39155, Western, ChIP, 1:4000, 0.03 μg/μL H3K27ac, Active motif, 39133, ChIP, 1:30 Total RNA Pol2, Millipore, 05-623, ChIP, 1:26 RNA Pol2 Ser5, Cell Signaling, 13523, ChIP, 1:43 RNA Pol2 Ser2, Cell Signaling, 13499, ChIP, 1:43 HP1, Cell Signaling, 2616, ChIP, 1:20 KDM3B, Cell Signaling, 3100, ChIP, 1:33.3 H3 Total Histone, Cell Signaling, 9715, Western, 1:5000 MYOG, Invitrogen, MA1-41042, Western, 1:500 Total PARP, Cell Signaling, 9542, Western, 1:1000 KDM1A, Cell Signaling,, 2184S, Western, 1:1000 KDM3B, Cell Signaling, 5377T, Western, 1:1000 KDM4B, Cell Signaling, 8639S, Western, 1:1000 KDM5A, Cell Signaling, 3876T, Western, 1:1000 Tubulin, Abcam, ab7291, Western, 1:5000 Actin-HRP, Santa Cruz, sc-47778, Western, 1:10000 GAPDH-HRP, Santa Cruz, sc-47724, Western, 1:10000 anti-mouse-HRP, Santa Cruz, sc-2005, Western, 1:5000 anti-rabbit-HRP, Santa Cruz, sc-2004, Western, 1:5000 Ki67, Invitrogen, MA5-14520, Flow Cytometry, 1:150 pH3, Biolegend, 650802, Flow Cytometry, 1:40 anti-rabbit-Alexa488, Invitrogen, A-11034, Flow Cytometry, 1:200 For the in-house PAX3-FOXO1 antibody, please refer the reference (Azorsa DO, et al. Mod Pathol, 2021, PMID: 33299109) for validation.
Chromatin Immunoprecipitation:Article Title: KDM3B inhibitors disrupt the oncogenic activity of PAX3-FOXO1 in fusion-positive rhabdomyosarcoma
Article Snippet: H3K9me2, Abcam, ab1220, Western, ChIP, 1:5000, 0.03 μg/μL H3K4me3, Active motif, 39159, Western, ChIP, 1:4000, 0.03 μg/μL H3K27me3, Active motif, 39155, Western, ChIP, 1:4000, 0.03 μg/μL H3K27ac, Active motif, 39133, ChIP, 1:30 Total RNA Pol2, Millipore, 05-623, ChIP, 1:26 RNA Pol2 Ser5, Cell Signaling, 13523, ChIP, 1:43 RNA Pol2 Ser2, Cell Signaling, 13499, ChIP, 1:43 HP1, Cell Signaling, 2616, ChIP, 1:20 KDM3B, Cell Signaling, 3100, ChIP, 1:33.3 H3 Total Histone, Cell Signaling, 9715, Western, 1:5000 MYOG, Invitrogen, MA1-41042, Western, 1:500 Total PARP, Cell Signaling, 9542, Western, 1:1000 KDM1A, Cell Signaling,, 2184S, Western, 1:1000 KDM3B, Cell Signaling, 5377T, Western, 1:1000 KDM4B, Cell Signaling, 8639S, Western, 1:1000 KDM5A, Cell Signaling, 3876T, Western, 1:1000 Tubulin, Abcam, ab7291, Western, 1:5000 Actin-HRP, Santa Cruz, sc-47778, Western, 1:10000 GAPDH-HRP, Santa Cruz, sc-47724, Western, 1:10000 anti-mouse-HRP, Santa Cruz, sc-2005, Western, 1:5000 anti-rabbit-HRP, Santa Cruz, sc-2004, Western, 1:5000 Ki67, Invitrogen, MA5-14520, Flow Cytometry, 1:150 pH3, Biolegend, 650802, Flow Cytometry, 1:40 anti-rabbit-Alexa488, Invitrogen, A-11034, Flow Cytometry, 1:200 For the in-house PAX3-FOXO1 antibody, please refer the reference (Azorsa DO, et al. Mod Pathol, 2021, PMID: 33299109) for validation.
Flow Cytometry:Article Title: KDM3B inhibitors disrupt the oncogenic activity of PAX3-FOXO1 in fusion-positive rhabdomyosarcoma
Article Snippet: H3K9me2, Abcam, ab1220, Western, ChIP, 1:5000, 0.03 μg/μL H3K4me3, Active motif, 39159, Western, ChIP, 1:4000, 0.03 μg/μL H3K27me3, Active motif, 39155, Western, ChIP, 1:4000, 0.03 μg/μL H3K27ac, Active motif, 39133, ChIP, 1:30 Total RNA Pol2, Millipore, 05-623, ChIP, 1:26 RNA Pol2 Ser5, Cell Signaling, 13523, ChIP, 1:43 RNA Pol2 Ser2, Cell Signaling, 13499, ChIP, 1:43 HP1, Cell Signaling, 2616, ChIP, 1:20 KDM3B, Cell Signaling, 3100, ChIP, 1:33.3 H3 Total Histone, Cell Signaling, 9715, Western, 1:5000 MYOG, Invitrogen, MA1-41042, Western, 1:500 Total PARP, Cell Signaling, 9542, Western, 1:1000 KDM1A, Cell Signaling,, 2184S, Western, 1:1000 KDM3B, Cell Signaling, 5377T, Western, 1:1000 KDM4B, Cell Signaling, 8639S, Western, 1:1000 KDM5A, Cell Signaling, 3876T, Western, 1:1000 Tubulin, Abcam, ab7291, Western, 1:5000 Actin-HRP, Santa Cruz, sc-47778, Western, 1:10000 GAPDH-HRP, Santa Cruz, sc-47724, Western, 1:10000 anti-mouse-HRP, Santa Cruz, sc-2005, Western, 1:5000 anti-rabbit-HRP, Santa Cruz, sc-2004, Western, 1:5000 Ki67, Invitrogen, MA5-14520, Flow Cytometry, 1:150 pH3, Biolegend, 650802, Flow Cytometry, 1:40 anti-rabbit-Alexa488, Invitrogen, A-11034, Flow Cytometry, 1:200 For the in-house PAX3-FOXO1 antibody, please refer the reference (Azorsa DO, et al. Mod Pathol, 2021, PMID: 33299109) for validation.
Biomarker Discovery:Article Title: KDM3B inhibitors disrupt the oncogenic activity of PAX3-FOXO1 in fusion-positive rhabdomyosarcoma
Article Snippet: H3K9me2, Abcam, ab1220, Western, ChIP, 1:5000, 0.03 μg/μL H3K4me3, Active motif, 39159, Western, ChIP, 1:4000, 0.03 μg/μL H3K27me3, Active motif, 39155, Western, ChIP, 1:4000, 0.03 μg/μL H3K27ac, Active motif, 39133, ChIP, 1:30 Total RNA Pol2, Millipore, 05-623, ChIP, 1:26 RNA Pol2 Ser5, Cell Signaling, 13523, ChIP, 1:43 RNA Pol2 Ser2, Cell Signaling, 13499, ChIP, 1:43 HP1, Cell Signaling, 2616, ChIP, 1:20 KDM3B, Cell Signaling, 3100, ChIP, 1:33.3 H3 Total Histone, Cell Signaling, 9715, Western, 1:5000 MYOG, Invitrogen, MA1-41042, Western, 1:500 Total PARP, Cell Signaling, 9542, Western, 1:1000 KDM1A, Cell Signaling,, 2184S, Western, 1:1000 KDM3B, Cell Signaling, 5377T, Western, 1:1000 KDM4B, Cell Signaling, 8639S, Western, 1:1000 KDM5A, Cell Signaling, 3876T, Western, 1:1000 Tubulin, Abcam, ab7291, Western, 1:5000 Actin-HRP, Santa Cruz, sc-47778, Western, 1:10000 GAPDH-HRP, Santa Cruz, sc-47724, Western, 1:10000 anti-mouse-HRP, Santa Cruz, sc-2005, Western, 1:5000 anti-rabbit-HRP, Santa Cruz, sc-2004, Western, 1:5000 Ki67, Invitrogen, MA5-14520, Flow Cytometry, 1:150 pH3, Biolegend, 650802, Flow Cytometry, 1:40 anti-rabbit-Alexa488, Invitrogen, A-11034, Flow Cytometry, 1:200 For the in-house PAX3-FOXO1 antibody, please refer the reference (Azorsa DO, et al. Mod Pathol, 2021, PMID: 33299109) for validation.
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